Internal standard how we have to used?
Internal standard
1. Quantities analysis
2. Purpose is
3. Selection process
4. Example is
Quantities analysis main three parts
• External standard
• Internal standard
• Standard addition
External standard
Here we find concentration of amount solution qualified compared response of standard and response sample
Internal standard
Addition of an alytes in a constant amount
to samples and standard during analysis.
Standard addition method
Addiion of analytes in a constant amount to samples to correct for matrix effects
When sample matrix abo contributes to the analytical signal
sample injection volumes
Purpose of Internal standard
To take care of error in sample injection volume in case HPLC /GC
To correct for the loss of analyte
during sample preparation such as,
• Poor extraction
• Poor dervatization
• Co-preciptation with other sample matrix
• Adsorpton on glasswware surface
• Uncontrolled evaporation during sample treatment
What is Internal standard in HPLC
During analysis consert amount of analytes mixed sample or standard call internal standard
Internal standard case Ratio of standard or sample is 1:1 are taken in general practice