Type of buffer in Hplc

1. Phosphate buffer
2. Citrate buffer
3. Formate buffer
4. Acetate buffer
5. Tris(hydrixymethyl) -aminomethane

Phosphate buffer

Pka 2.1

Ph range 1.1 tob3. 1

Pka 7.2

Ph range 6.2 to 8.3

Pk 12.3

Ph range 11.3 to 13.3

Citrate

Pka 3.1

Ph range 2.1 to 4.1

Pka 4.7

Ph 3.7 to 5.7

Pka 5.4

Ph range 4.4 to 6.4

Format

Pka 3.8

Ph range 2.8 to 4.8

Acetate

Pka 4.8

Ph range 3.8 to 5.8

 

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