HPLC PUMP TROUBLESHOOTING

HPLC (High-Performance Liquid Chromatography) pump issues can significantly impact the performance and reliability of your chromatographic analysis. Here are some common problems and potential solutions: Common Issues: Flow rate inconsistencies: Variations in flow...

HPLC calibration compound might be use

B – naphtholCaffeineToluene Whu this use • Not harm your column • Easy mixtures like methanol water mix use the mobile phase • So not harm your column • Peak are very identical Not use any buffer so not harm in column Share this: Click to share on Facebook...

HPLC calibration which substance use why ?

B – naphtholCaffeineToluene Whu this use • Not harm your column • Easy mixtures like methanol water mix use the mobile phase • So not harm your column • Peak are very identical Not use any buffer so no harm in column Share this: Click to share on Facebook (Opens...

Chromatography 621 number chapter adjustment parameters is 8

1) Ph
2) injection volume
3) Temperature
4) buffer Concen
5) Column
6) Mobile phase ratio
7) Wave leny
8)Flow rate

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FACTOR AFFECTS ON HPLC COLUMN

1) Partial Blockage of column 2) Adsorption of sample impurities inside column surface. 3) Poorly packed stationary phase. 4) Mechanical or thermal shocks during Handling. 5) Chemical attack on the stationary phase. Share this: Click to share on Facebook (Opens in new...

HPLC Interview Top 15 Question and answer

1. What is HPLC? Answer:HPLC stands for High-Performance Liquid Chromatography. It is a technique used for separating, identifying, and quantifying components in a mixture. It uses a liquid mobile phase under high pressure to pass through a column containing a...