by maheshbhuva | Mar 26, 2026 | Analytical Chemistry, Analytical method development hplc,, Hplc, Hplc column, Pharmaceutical interview,, Quality control (QC)
HPLC column HPLC columns are classified in the United States Pharmacopeia (USP) under the “L” designation system, with each type assigned a specific USP code (e.g., L1, L3, L7). The main USP chapter that governs chromatography, including HPLC columns, is USP...
by maheshbhuva | Mar 25, 2025 | Hplc, Hplc column, HPLC VIAL
B – naphtholCaffeineToluene Whu this use • Not harm your column • Easy mixtures like methanol water mix use the mobile phase • So not harm your column • Peak are very identical Not use any buffer so no harm in column Share this: Click to share on Facebook (Opens...
by maheshbhuva | Feb 20, 2025 | Hplc, Hplc column
1) Partial Blockage of column 2) Adsorption of sample impurities inside column surface. 3) Poorly packed stationary phase. 4) Mechanical or thermal shocks during Handling. 5) Chemical attack on the stationary phase. Share this: Click to share on Facebook (Opens in new...
by maheshbhuva | May 4, 2024 | Hplc, Hplc column, Quality control (QC)
Theoretical Plates chromatography In chromatography, theoretical plates are a fundamental concept used to assess the separation efficiency of a chromatographic column. They play a crucial role in determining the quality and effectiveness of the chromatographic...
by maheshbhuva | Mar 31, 2024 | Analytical Chemistry, Analytical method development hplc,, Hplc, Hplc column, Quality control (QC)
In HPLC chromatography, the column is where the separation of the sample takes place. This is where the sample is passed through the stationary phase with the mobile phase, resulting in the separation of the sample components. As the sample being analyzed is likely to...
by maheshbhuva | Dec 22, 2022 | Hplc, Hplc column
1) Partial Blockage of column 2) Adsorption of sample impurities inside column surface 3) Poorly packed stationary phase. 4) Mechanical or thermal shocks during Handling. 5) Chemical attack on the stationary phase. Quality control (Q.C) What is Internal standard HPLC...
