HPLC calibration which substance use why ?

B – naphtholCaffeineToluene Whu this use • Not harm your column • Easy mixtures like methanol water mix use the mobile phase • So not harm your column • Peak are very identical Not use any buffer so no harm in column Share this: Click to share on Facebook (Opens...

FACTOR AFFECTS ON HPLC COLUMN

1) Partial Blockage of column 2) Adsorption of sample impurities inside column surface. 3) Poorly packed stationary phase. 4) Mechanical or thermal shocks during Handling. 5) Chemical attack on the stationary phase. Share this: Click to share on Facebook (Opens in new...