HPLC Calibration we have perform as earlier as per protocol Company will provided Like example Shimanzu HPLC calibration will perform as per that guidance’s
But now a day In 16th September 2021 As per IPC Pharmacopeia give guidance(Document ID IPC/GD/05)
HPLC Calibration

hplc instrument
AS per guideline
- Calibration of pump
- Calibration of Autosampler
- Calibration Column Oven
- Calibration of Detector
Calibration of pump
- Flow rate accuracy
- Flow rate consistency
- Compositional accuracy (gradient profile)
- Delay volume of system
Calibration of Autosampler
- Injection volume accuracy
- Injection volume precosion
- Injection volume linearity
- Auto sampler temperature accuracy
Calibration of Column Oven
Calibration of Detector
Calibration of detector shall be done on the basis of the following parameters:
- Detector linearity
- Â Wavelength accuracy