Fluctuating retention times Unstable Pressure, check pressure section If mobile phase is not pre-mixed, there could be problem with the ratio been properly mixed(Inconsistent on-line mobile phase mixing) Unstable Column temperature Increasing Retention Times Decreasing Flow rate Decreasing column temperature Decreasing Retention Times Increasing flow rate Increasing column tempera HPLC troubleshooting related RT
HPLC troubleshooting related RT
