-
Detector wavelength : Adjustment not permitted
- Defined Sensitivity of MP
- Change wave length some IMP may go missing
- Â Responses might be different
-
Stationary phase: No change (eg not replacement C18 b C8 )
- Change RT
- Change Selectivity
- Change Elution respectively peck
- RRT change RRF change miss identified IMP
-
Particle size for packed column (In case GC): Maximum reduction of 50% ;
- No Increase permitted
- If increase particle size surface of column increase so poor RT meet hence not increase particle size
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