• Detector wavelength : Adjustment not permitted

  • Defined Sensitivity of MP
  • Change wave length some¬† IMP may go missing
  • ¬†Responses might be different
  1. Stationary phase: No change (eg not replacement C18 b C8 )

    • Change RT
    • Change Selectivity
    • Change Elution respectively peck
    • RRT change RRF change miss identified IMP
  2. Particle size for packed column (In case GC): Maximum reduction of 50% ;

    • No Increase permitted
    • If increase particle size surface of column increase so poor RT meet hence not increase particle size