by maheshbhuva | Jan 20, 2023 | Analytical Chemistry, Analytical method development hplc,, Hplc
Chromatography is a method used to separate and analyze mixtures of compounds. There are several different types of chromatography, including gas chromatography, liquid chromatography, and paper chromatography. Each type uses a different method to separate the...
by maheshbhuva | Dec 28, 2022 | Analytical Chemistry, Analytical method development hplc,, Hplc
it is miscible with water, has good eluotropic strength and also low viscosity alone and mixed with water. Acetonitrile high-sensitivity analysis with UV detection in the short-wavelength region. However , organic solvents which have been processed for HPLC IN...
by maheshbhuva | Dec 25, 2022 | Analytical Chemistry, Hplc, Method validation
Accuracy is also part of method validation different place Different terminology used for accuracy like recovery, truth value, biaus etc Accuracy mainly defined Result of testing compound how much near by truth value. Example: take azithromycin tablet 500 mg Assume...
by maheshbhuva | Dec 25, 2022 | Hplc, Method validation
It t part of analytical method validation Definition: Specificity to defined capability to assess unequivocally the analyte in the presence of components which may be expected to be present. They might be impurities, degradants, matrix, etc. Here we identify that Any...
by maheshbhuva | Dec 22, 2022 | Hplc, Hplc column
1) Partial Blockage of column 2) Adsorption of sample impurities inside column surface 3) Poorly packed stationary phase. 4) Mechanical or thermal shocks during Handling. 5) Chemical attack on the stationary phase. Quality control (Q.C) What is Internal standard HPLC...
by maheshbhuva | Dec 14, 2022 | Gc, Hplc
Internal standard how we have to used? Internal standard 1. Quantities analysis 2. Purpose is 3. Selection process 4. Example is Quantities analysis main three parts • External standard • Internal standard • Standard addition External standard Here we find...
